Process optimization in the extract of perilla seed oil with plant protein hydrolysate complex enzyme

Perilla seed oil riched in the ω-3 fatty acid especially α-linolenic acid, is associated with a beneficial role human health. In present study, Aqueous Enzymatic Extraction (AEE) of crude from seeds was investigated. The extraction parameters including enzyme type, solid-to-water ratio, pH, temperature, loading and hydrolysis time were determined by Response Surface Methodology (RSM). RSM results showed optimum for plant protein hydrolysate complex enzyme. optimized values loading, extracting perilla respectively 1:4, 6, 55 °C, 2% 4.5 h. Under conditions, maximum recovery 88.52%, extracted AEE possessed high yield.